In Situ Hybridization Protocols By Nicoletta Archidiacono, Rachele Antonacci, Antonino Forabosco, Mariano Rocchi (auth.), K. H. Andy Choo (eds.)
1995 | 483 Pages | ISBN: 0896032809 | PDF | 18 MB
Thirty-one detailed, practical chapters many by the originators themselves present easy-to-follow protocols of in situ hybridization techniques for mapping DNA sequences onto chromosomes, quantitation/localization of RNA in tissues, and detection of virus nucleic acids. The methods serve molecular biologists in most areas of basic and applied research, and in every sort of organism ranging from viruses to Drosophila to humans. The expert contributors provide step-by-step instructions for the preparation of probes for DNA, teleomeric repeats, cDNA sequences, small synthetic oligomers, whole cosmids, mega-size YACs, and chromosome-specific paints from somatic cell hybrids and flow-sorted chromosomes. They also present radioactive and nonradioactive probe labeling procedures with the use of nick translation, random priming, multicolor labeling, PRINS labeling of DNA and RNA, and PCR in situ hybridization. Color illustrations enhance the text. Troubleshooting tips, alternative ways of doing things, and informative explanations of why certain steps are done -- aids not usually found in standard journal recipes -- guarantee a significant difference in the outcome of experiments.

Impact of COVID-19 on Emerging Contaminants : One Health Framework for Risk Assessment and Remediation
by Manish Kumar and Sanjeeb Mohapatra
English | 2022 | ISBN: 9811918465 | 438 Pages | True ePUB | 39 MB

I'm More Dateable than a Plate of Refried Beans: And Other Romantic Observations by Ginny Hogan
English | May 3, 2022 | ISBN: 1797212265 | True EPUB | 240 pages | 59.7 MB
From comedian Ginny Hogan, this laugh-out-loud collection of humor observations explores all the ups and downs of modern romance.

How to Publish in Biological Sciences: A Guide for the Uninitiated
English | 2023 | ISBN: 1032116447 | 278 Pages | PDF (True) | 12.3 MB
This book is a guide specifically for Early Career Researchers on how to publish in the Biological Sciences, whether that be your first manuscript or if you're already experienced - there's something for everyone.

James Braha, "How to Be a Great Astrologer: The Planetary Aspects Explained"
English | ISBN: 0935895027 | | 256 pages | EPUB | 475 KB
New, from the author of ANCIENT HINDU ASTROLOGY FOR THE MODERN WESTERN ASTROLOGER, a hard hitting, plain spoken guide to interpreting the planetary aspects in Western astrology. Braha pulls no punches in this extremely clear, accessible reference text. It is the most comprehensive work on the subject to date, & includes 23 descriptive pages on the Moon s nodes in the signs & houses. Exquisitely designed, with gold embossed cover & 21 full size Gustave Dore illustrations. DIAMOND FIRE ASTROLOGY MAGAZINE SAYS: "This book is more than just another well written book on aspects. It is a work of art. The book is lavishly illustrated with angelic, celestial pictures. And it includes quotes & aphorisms from the great figures of this age. The whole effect is to put the reader in an exalted frame of mind - a frame of mind where real learning & inspiration take place. The art alone is worth the price of the book. The aspect delineations are well written & astrologically accurate. The section on the nodes is one of the clearest explanations I have ever seen in print!" Invaluable to professionals & amateurs alike.

How to Be a Bad Emperor : An Ancient Guide to Truly Terrible Leaders
by Suetonius and Josiah Osgood
English | 2020 | ISBN: 0691193991 | 312 Pages | True AZW3 | 0.25 MB

How to Be Free : An Ancient Guide to the Stoic Life
by Epictetus and Anthony Long
English | 2018 | ISBN: 0691177716 | 232 Pages | True AZW3 | 0.28 MB

Hemostasis and Thrombosis Protocols By K. John Pasi (auth.), David J. Perry, K. J. Pasi (eds.)
1999 | 368 Pages | ISBN: 0896034194 | PDF | 3 MB
In Hemostasis and Thrombosis Protocols, leading experimentalists describe in detail their proven, cutting-edge methods for research on hemostasis and thrombosis, as well as their diagnostics. The techniques range from widely used basic assays to methods that are specialized for mutational analysis and specific disorders. Readily reproducible, these powerful methods can be used to screen for such inherited disorders as hemophilia A and B, von Willebrands Disease, Factor XI and antithrombin deficiency, protein S deficiency, factor V Leiden mutation, and other problems. The protocols reflect the many major advances that have occurred in our understanding of the molecular genetics of normal hemostasis and it variants, as well as of the coagulation cascade itself. Suitable for both diagnostic and research laboratories, Hemostasis and Thrombosis Protocols enables experienced and novice investigators alike readily to master the complexities of research on molecular hemostasis and thrombosis, as well as work toward the development of productive new molecular diagnostics.

English | ISBN: 141146981X | 2014 | 96 pages | EPUB | 279 KB
When an essay is due and dreaded exams loom, this title offers students what they need to succeed. It provides chapter-by-chapter analysis, explanations of key themes, motifs and symbols, a review quiz, and essay topics. It is suitable for late-night studying and paper writing.

Greg Behrendt, "He's Just Not That Into You: The No-Excuses Truth to Understanding Guys"
English | ISBN: 141690977X | 2009 | 208 pages | EPUB | 561 KB
He's Just Not That Into You-based on a popular episode of Sex and the City-is tough love advice for otherwise smart women on how to tell when a guy just doesn't like them enough, so they can stop wasting time making excuses for a dead-end relationship. It's the best relationship advice you'll ever receive.