Coupled Bioluminescent Assays: Methods, Evaluations, and Applications By Michael J. Corey(auth.)
2008 | 315 Pages | ISBN: 0470108835 | PDF | 12 MB
This book highlights the applications of coupled bioluminescence assay techniques to real-world problems in drug discovery, environmental and chemical analysis, and biodefense. It separates theoretical aspects from the applied sections in a clear and readable way. Coupled Bioluminescent Assays, explains the uses of CB technologies across drug discovery to analyze toxicity, drug receptors, and enzymes. It covers applications in environmental analysis and biodefense, including cytotoxicity, fertilizer and explosives analysis, and nerve agent and pesticide detection. This is the premier reference on coupled bioluminescent assays for chemists, biochemists, and molecular biologists.Content: Chapter 1 Introduction (pages 1-23): Chapter 2 Coupled Bioluminescent Reactions in Practice (pages 24-55): Chapter 3 Coupled Bioluminescent Cytotoxicity Assays (pages 57-84): Chapter 4 The Role of Coupled Bioluminescent Assays in Kinase Screening and Study (pages 85-102): Chapter 5 Coupled Bioluminescent Phosphatase Assays (pages 103-130): Chapter 6 Acetylcholinesterase (pages 131-138): Chapter 7 Measurement of Nitric Oxide Synthase Activity by Coupled Bioluminescence (pages 139-149): Chapter 8 The Coupled Bioluminescent Pyrophosphorolysis Assay (pages 150-159): Chapter 9 Coupled Luminescent Assays of G?Protein?Coupled Receptors (pages 160-190): Chapter 10 Coupled Bioluminescent Protease Assays (pages 191-200): Chapter 11 Coupled Luminescent Assays Involving Aequorin (pages 201-208): Chapter 12 Coupled Bioluminescent Reporter Assays (pages 209-217): Chapter 13 Coupled Bioluminescent Assays: Regulatory Concerns (pages 218-240): Chapter 14 Coupled Bioluminescent Determination of Bioburden and Sterility (pages 241-246): Chapter 15 Environmental Applications of Coupled Bioluminescent Assays (pages 247-258):

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